Effects of carbenicillin and cefotaxime on callus growth and somatic embryogenesis from adventitious roots of papaya
نویسندگان
چکیده
Carbenicillin and cefotaxime, two antibiotics commonly used for excluding Agrobacterium tumefaciens during plant transformation, were tested for their bacteriostatic effects as well as for their effects on plant regeneration in adventitious root explants of papaya following co-culture with Agrobacterium. A washing step with sterilized distilled water two days after co-culture enhanced the bacteria-suppressing effects of antibiotics. Proliferation of Agrobacterium was completely suppressed in the medium containing 125 mgl carbenicillin or cefotaxime. Callus fresh weight increase was apparently enhanced in the media with higher concentrations of carbenicillin (250-500 mgl), but was extremely inhibited in media with the same concentrations of cefotaxime. Higher percentages of somatic embryos were found in the medium with 125 mgl carbenicillin or 250 mgl cefotaxime; however larger numbers of somatic embryos from the individual callus were obtained in the medium with 125 mgl carbenicillin than in the medium with 250 mgl cefotaxime. Percentages of abnormal somatic embryos were lower in the medium with lower concentrations of carbenicillin (125-250 mgl). Favorable conditions for use of the two antibiotics for suppressing bacteria growth and enhancing regeneration of papaya plantlets from adventitious roots are discussed.
منابع مشابه
Development of Transgenic Papaya through Agrobacterium-Mediated Transformation
Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and β-glucuronidase (GUS) as the reporter gene. The explants were co-cultivated with Agrobacterium tumefaciens on regeneration medium ...
متن کاملInduction of Callus and Somatic Embryogenesis from Cotyledon Explants of Fagonia indica Burm.
To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for cal...
متن کاملSomatic Embryogenesis and Plant Regeneration in Evening Primrose (Oenothera biennis L.)
Callus induction responses and regeneration through callus-mediated SE were studied from evening primrose (Oenothera biennis L.) as an important medicinal plant of Onagraceae mainly known for its gamma-linoleic acid (GLA) content. The effects of cytokinins, 6-Benzylaminopurine (BAP) and N-(2-furfurylamino)1-H-purine-6-amine [Kinetin (Kin)] and their concentrations (0, 0.5, 1.0 and 1.5 mg/l) in ...
متن کاملSomatic embryogenesis and plant regeneration from immature embryo explant of papaya (Carica papaya L. cv. washington and honey dew).
Immature zygotic embryo explants of Carica papaya were cultured on MS medium supplemented with 2,4-D (2.0 mg/l) and formed globular embryos on explants without callus formation in 4-6 weeks. Maturation and conversion of somatic embryos was also achieved on the same medium. Cotyledonary stage embryos germinated to 63.66 and 68.33% in cv. honey dew and washington respectively in MS basal medium s...
متن کاملPlant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic ca...
متن کامل